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Goat Anti-MNSOD (aa119-130) Antibody (EB10972)

Related Products: EBP10971 - Immunizing Peptide, EBP10972 - Immunizing Peptide, Goat Anti-MNSOD (isoform A) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10972 Goat Anti-MNSOD (aa119-130) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10972 1
EB10972 (0.3µg/ml) staining of Human Cerebellum (A), Mouse Brain (B), Rat Brain (C) and Pig Brain (D) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10972 2
EB10972 (0.1µg/ml) staining of HeLa, HepG2, HEK293 and NIH3T3 (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10972 3
EB10972 (0.01µg/ml) staining of Mouse (A) and Rat (B) Spinal Cord lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10972 4
EB10972 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SOD2, superoxide dismutase 2, mitochondrial, RP1-56L9.2, IPOB, MNSOD, MVCD6, Mn superoxide dismutase, indophenoloxidase B, manganese-containing superoxide dismutase, superoxide dismutase [Mn], mitochondrial
Official Symbol: SOD2
Accession Number(s): NP_000627.2; NP_001019637.1
Human GeneID(s): 6648
Non-Human GeneID(s): 20656 (mouse), 24787 (rat)
Important Comments: NB: The immunizing peptide represents the acetylation site including K122 according to isoform A. This antibody is expected to recognize both reported isoforms (NP_000627.2; NP_001019637.1) . Reported variants represent identical protein: NP_000627.2; NP_001019637.1.

Immunogen

Peptide with sequence C-EAIKRDFGSFDK, from the internal region of the protein sequence according to NP_000627.2; NP_001019637.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 24kDa band observed in Human Brain (cerebellum) lysates and in Mouse and Rat Brain and Spinal Cord lysates, while approx 26kDa band was observed in lysates of cell lines HeLa, HepG2, HEK293 and NIH3T3 (calculated MW of 24.8kDa according to Human NP_000627.2, and 24.6kDa according to Mouse NP_038699.2). Recommended concentration: 0.01-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish

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