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Goat Anti-MYF5 Antibody (EB08504)

Related Products: EBP08504 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08504 Goat Anti-MYF5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08504 1
EB08504 (0.5µg/ml) staining of Muscle lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MYF5, myogenic factor 5
Official Symbol: MYF5
Accession Number(s): NP_005584.1
Human GeneID(s): 4617

Immunogen

Peptide with sequence PECNSPVWSRKSST, from the internal region of the protein sequence according to NP_005584.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 28kDa band observed in Human Skeletal Muscle lysates (calculated MW of 28.4kDa according to NP_005584.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of 26kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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