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Goat Anti-MYH9 Antibody (EB09020)

Related Products: EBP09020 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09020 Goat Anti-MYH9 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09020 1
EB09020 (0.3µg/ml) staining of Human peripheral blood lymphocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09020 2
EB09020 (1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: myosin, heavy chain 9, non-muscle, RP1-68O2.1, DFNA17, EPSTS, FTNS, MGC104539, MHA, NMHC-II-A, NMMHCA, MYH9 variant protein, OTTHUMP00000028706, cellular myosin heavy chain, type A, myosin, heavy polypeptide 9, non-muscle, non-muscle myosin heavy chain, non-muscle myosin heavy polypeptide 9, nonmuscle myosin heavy chain II-A
Official Symbol: MYH9
Accession Number(s): NP_002464.1
Human GeneID(s): 4627

Immunogen

Peptide with sequence C-DQINTDLNLERSH, from the internal region of the protein sequence according to NP_002464.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 250kDa band observed in lysates of Human Peripheral Blood Mononucleocytes and of NIH3T3 (calculated MW of 227kDa according to Human NP_002464.1 and to Mouse NP_071855.2). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in Western blot on Human:
Harms RZ, Borengasser K, Kumar V, Sarvetnick N
Anti-human Interleukin (IL)-4 Clone 8D4-8 Cross-Reacts With Myosin-9 Associated With Apoptotic Cells and Should Not Be Used for Flow Cytometry Applications Querying IL-4 Expression.
Front Cell Dev Biol. 2019 Apr 9;7:46.
PMID: 31024909

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