Change country
Home

Goat Anti-MYO1B Antibody (EB10549)

Related Products: EBP10549 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10549 Goat Anti-MYO1B Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10549 1
EB10549 (5µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB10549 2
EB10549 (1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MYO1B, myosin IB, myr1, MMI-alpha, MMIa, MYH-1c, MYO1B variant protein, OTTHUMP00000165861, myosin I alpha, myosin-Ib
Official Symbol: MYO1B
Accession Number(s): NP_001123630.1; NP_036355.2
Human GeneID(s): 4430
Non-Human GeneID(s): 17912 (mouse), 117057 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001123630.1; NP_036355.2). Reported variants represent identical protein: NP_001123630.1, NP_001155291.1.

Immunogen

Peptide with sequence C-RTVEAKQEKVSTT, from the internal region of the protein sequence according to NP_001123630.1; NP_036355.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 120kDa band observed in lysates of cell line NIH3T3 (calculated MW of 125kDa according to Human NP_036355.2 and 128kDa according to Mouse NP_034993.2). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in the neuronal bodies. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Product Reviews

Please login to review this product.