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Goat Anti-NANOG (C Terminus) Antibody (EB07693)

Code Name Applications Availability Size Tested species Price Grade  
EB07693 Goat Anti-NANOG (C Terminus) Antibody Pep-ELISA, WB, IHC, EIA Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07693 1
EB07693 (2µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07693 2
EB07693 (0.05µg/ml) staining of HeLa (nuclear) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07693 3
EB07693 (5ug/ml) as the reporter with EB002009 as the capture rabbit antibody (5ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: NANOG, Nanog homeobox, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
Official Symbol: NANOG
Accession Number(s): NP_079141.2
Human GeneID(s): 79923

Immunogen

Peptide with sequence C-DLFLNYSMNMQPED, from the C Terminus of the protein sequence according to NP_079141.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1µg/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources.
IHC: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2µg/ml.

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NANOG captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml. This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB06860.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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