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Code Name Applications Availability Size Tested species Price Grade  
EB08947 Goat Anti-NCE2 / UBE2F Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08947 1
EB08947 (0.05µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08947 2
In paraffin embedded Human Placenta shows membrane staining in trophoblasts. Recommended concentration: 2.5-3.8µg/ml.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 conjugating enzyme, NEDD8-conjugating enzyme
Official Symbol: UBE2F
Accession Number(s): NP_542409.1
Human GeneID(s): 140739
Non-Human GeneID(s): 67921 (mouse), 363284 (rat)

Immunogen

Peptide with sequence C-HLRDKEDFRNKVDD, from the C Terminus of the protein sequence according to NP_542409.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 20kDa band observed in Mouse Liver lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and to Mouse NP_080730.1). Recommended concentration: 0.05-0.2µg/ml.
IHC: In paraffin embedded Human Placenta shows membrane staining in trophoblasts-. Recommended concentration: 2.5-3.8µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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