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Goat Anti-NCKAP1 Antibody (EB11695)

Related Products: EBP11695 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11695 Goat Anti-NCKAP1 Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11695 1
EB11695 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB11695 2
EB11695 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB11695 3
EB11695 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: FLJ11291, HEM2, KIAA0587, membrane-associated protein HEM-2, MGC8981, NAP 1, NAP1, NAP125, NCKAP1, NCK-associated protein 1, OTTHUMP00000163460, OTTHUMP00000163461, p125Nap1
Official Symbol: NCKAP1
Accession Number(s): NP_038464.1; NP_995314.1
Human GeneID(s): 10787
Non-Human GeneID(s): 50884 (mouse), 58823 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_038464.1; NP_995314.1).

Immunogen

Peptide with sequence C-TPREYLTSHLEIR, from the internal region of the protein sequence according to NP_038464.1; NP_995314.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 100kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5µg/ml antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calculated MWt.is 129kDa according to NP_038464.1 and approx. 110-125kDa bands have been observed by other sources.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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