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Goat Anti-NDUFS2 Antibody (EB10189)

Related Products: EBP10189 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10189 Goat Anti-NDUFS2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10189 1
EB10189 (0.1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: complex 1, mitochondrial respiratory chain, 49-KD subunit, NADH dehydrogenase (ubiquinone) Fe-S protein 2, NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase), NADH-ubiquinone oxidoreductase NDUFS2 subunit, NDUFS2
Official Symbol: NDUFS2
Accession Number(s): NP_004541.1; NP_001159631.1
Human GeneID(s): 4720
Non-Human GeneID(s): 226646 (mouse), 289218 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004541.1; NP_001159631.1).

Immunogen

Peptide with sequence C-SRGVRQWQPDVE, from the internal region of the protein sequence according to NP_004541.1; NP_001159631.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 48kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 51.9kDa according to NP_001159631.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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