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Goat Anti-NDUFS6 Antibody (EB09788)

Related Products: EBP09788 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09788 Goat Anti-NDUFS6 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc
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EB09788 1
EB09788 (0.01µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09788 2
EB09788 (0.1µg/ml) staining of Mouse (A) and Rat (B) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09788 3
EB09788 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase), NDUFS6
Official Symbol: NDUFS6
Accession Number(s): NP_004544.1
Human GeneID(s): 4726
Non-Human GeneID(s): 407785 (mouse), 29478 (rat)

Immunogen

Peptide with sequence C-RIRFVGRQKEVNEN, from the internal region of the protein sequence according to NP_004544.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 15kDa band observed in Human Heart lysates and 14kDa in Mouse and Rat Heart lysates (calculated MW of 13.7kDa according to NP_004544.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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