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Goat Anti-NDUFS8 Antibody (EB10194)

Related Products: EBP10193 - Immunizing Peptide, EBP10194 - Immunizing Peptide, Goat Anti-NDUFS8 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10194 Goat Anti-NDUFS8 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10194 1
EB10194 (0.1µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CI23KD, complex I-23kD, NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase), NADH dehydrogenase ubiquinone Fe-S 8, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY, NDUFS8
Official Symbol: NDUFS8
Accession Number(s): NP_002487.1
Human GeneID(s): 4728
Non-Human GeneID(s): 225887 (mouse), 293652 (rat)

Immunogen

Peptide with sequence C-AEPRADGSRR, from the Internal region of the protein sequence according to NP_002487.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 25kDa band observed in Human and Mouse Heart lysates (calculated MW of 23.7kDa according to NP_002487.1). Recommended concentration: 0.1-0.3µg/ml. An additional fainter band of 90kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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