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Goat Anti-NEFM / NF-M Antibody (EB09641)

Related Products: EBP09641 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09641 Goat Anti-NEFM / NF-M Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09641 1
EB09641 (0.03µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09641 2
EB09641 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NEFM, neurofilament, medium polypeptide, NEF3, NF-M, NFM, neurofilament triplet M protein, neurofilament, medium polypeptide 150kDa, neurofilament-3 (150 kD medium)
Official Symbol: NEFM
Accession Number(s): NP_005373.2
Human GeneID(s): 4741
Non-Human GeneID(s): 18040 (mouse), 24588 (rat)
Important Comments: This antibody is expected to recognize reported isoform 1 (NP_005373.2) only.

Immunogen

Peptide with sequence C-QASHITVERKDYLK, from the internal region of the protein sequence according to NP_005373.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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