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Code Name Applications Availability Size Tested species Price Grade  
EB10519 Goat Anti-NEK7 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10519 1
EB10519 (0.05µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10519 2
EB10519 (3.75µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NIMA (never in mitosis gene a)-related kinase 7, NIMA-related kinase 7, NEK7
Official Symbol: NEK7
Accession Number(s): NP_598001.1
Human GeneID(s): 140609
Non-Human GeneID(s): 59125 (mouse), 360850 (rat)

Immunogen

Peptide with sequence C-NTLANFRIEKK, from the internal region of the protein sequence according to NP_598001.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 35kDa band observed in lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_598001.1). Recommended concentration: 0.05-0.2µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Thyroid. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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