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Goat Anti-Neurochondrin Antibody (EB07600)

Related Products: EBP07600 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07600 Goat Anti-Neurochondrin Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07600 1
EB07600 (0.3µg/ml) staining of HepG2 (A) and (1ug/ml) U251 cell lysate (B) + peptide (C) (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NCDN, neurochondrin, KIAA0607
Official Symbol: NCDN
Accession Number(s): NP_055099.1; NP_001014841.1
Human GeneID(s): 23154
Non-Human GeneID(s): 26562 (mouse), 89791 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_055099.1; NP_001014841.1). Reported variants represent identical protein (NP_055099.1; NP_001014839.1).

Immunogen

Peptide with sequence C-HYLLQVGSEKQKEP, from the internal region of the protein sequence according to NP_055099.1; NP_001014841.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75kDa band observed in lysates of cell lines HepG2 and U251 (calculated MW of 77.2kDa according to NP_001014841.1). An additional band of unknown identity was also observed in U251 lysates at 55kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Kidney and Brain and NIH3T3 cell line for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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