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Goat Anti-Neurogranin precursor, Biotinylated Antibody (EB09686-B)

Related Products: EBP09686-B - Immunizing Peptide, EBP09686 - Immunizing Peptide, Goat Anti-Neurogranin precursor Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09686-B Goat Anti-Neurogranin precursor, Biotinylated Antibody Pep-ELISA, WB, Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09686-B 1
Biotinylated EB09686 (0.1µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NRGN, neurogranin, RC3, hng, calmodulin-binding protein, neurogranin (protein kinase C substrate, RC3), ng, protein kinase C substrate
Official Symbol: NRGN
Accession Number(s): NP_006167.1
Human GeneID(s): 4900
Important Comments: Reported variants represent identical protein: NP_006167.1, NP_001119653.1

Immunogen

Peptide with sequence CKIKSGERGRKG., from the internal region of the protein sequence according to NP_006167.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 16kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 7.6kDa according to NP_006167.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274(3):1294-300; PMID: 9880498). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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