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Goat Anti-Neuropeptide Y receptor Y5 Antibody (EB06769)

Code Name Applications Availability Size Tested species Price Grade  
EB06769 Goat Anti-Neuropeptide Y receptor Y5 Antibody Pep-ELISA, WB, IP, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06769 1
EB06769 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06769 2
EB06769 (3.8µg/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: neuropeptide Y receptor Y5, HGNC:7958, NPY5R, NPYR5, NPY5-R, NPY-Y5 receptor, NPYY5-R, Y5 receptor
Official Symbol: NPY5R
Accession Number(s): NP_006165.1
Human GeneID(s): 4889
Non-Human GeneID(s): 18168 (mouse), 25340 (rat)

Immunogen

Peptide with sequence C-GLSNKENRLEEN, from the internal region of the protein sequence according to NP_006165.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7kDa according to NP_006165.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration, 4-6µg/ml.

Immunoprecipitation: Customer feedback showed specific results when this product was used in IP.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody has been successfully used in WB and IP on CHO-K1 cells:
Magdalena Czarneckaa, Congyi Lua,b, Jennifer Ponsa, Induja Maheswarana, Pawel Ciborowskic, Lihua Zhangd, Amrita Cheemad, Joanna Kitlinskaa
Neuropeptide Y receptor interactions regulate its mitogenic activity
Neuropeptides. 2019 Feb;73:11-24. 
PMID: 30503694

This antibody has been successfully used in Western blot on Human:
Czarnecka M, Kitlinska J.
Cell Surface Protein Detection to Assess Receptor Internalization.
Bio Protoc. 2016 Oct 20;6(20)
PMID: 28413811

This antibody has been successfully used in Western blot on Human:
Sheriff S, Ali M, Yahya A, Haider KH, Balasubramaniam A, Amlal H.
Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line.
Mol Cancer Res. 2010 Apr;8(4):604-14.
PMID: 20332211

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