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Goat Anti-Neuroserpin Antibody (EB06688)

Related Products: EBP06688 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06688 Goat Anti-Neuroserpin Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06688 1
EB06688 (1µg/ml) staining of Kelly (A) and K562 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06688 2
EB06688 (1µg/ml) staining of Human Placenta (A), Mouse Brain (B) and (0.5ug/ml) Rat Braun (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06688 3
EB06688 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06688 4
EB06688 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: neuroserpin, SERPINI1, PI12, serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1, protease inhibitor 12 (neuroserpin), serpin peptidase inhibitor, clade I (neuroserpin), member 1, DKFZp781N13156
Official Symbol: SERPINI1
Accession Number(s): NP_005016.1
Human GeneID(s): 5274
Important Comments: Reported variants represent identical protein (NP_005016.1; NP_001116224.1).

Immunogen

Peptide with sequence C-TMNTSGHDFEEL, from the C Terminus of the protein sequence according to NP_005016.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48kDa band observed in lysates of cell lines Kelly and K562 and in Human Placenta and Mouse and Rat Brain lysates (calculated MW of 46.4kDa according to Human NP_005016.1, Mouse NP_033276.1 and Rat NP_446231.1). Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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