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Goat Anti-NFATC3 / NFAT4 Antibody (EB09504)

Related Products: EBP09504 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09504 Goat Anti-NFATC3 / NFAT4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09504 1
EB09504 (0.03µg/ml) staining of Jurkat nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NFATC3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3, NFAT4, NFATX, T cell transcription factor NFAT4, nuclear factor of activated T-cells c3 isoform IE-Xa, nuclear factor of activated T-cells, cytoplasmic 3
Official Symbol: NFATC3
Accession Number(s): NP_775188.1; NP_004546.1; NP_775186.1; NP_775187.1
Human GeneID(s): 4775
Important Comments: This antibody is expected to recognize all four reported isoforms (NP_775188.1; NP_004546.1; NP_775186.1; NP_775187.1).

Immunogen

Peptide with sequence C-HQELDAHEDDLQIN, from the internal region (near N Terminus) of the protein sequence according to NP_775188.1; NP_004546.1; NP_775186.1; NP_775187.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 150kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 116kDa according to NP_775188.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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