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Goat Anti-NIR1 / PITPNM3 Antibody (EB06334)

Related Products: EBP06334 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06334 Goat Anti-NIR1 / PITPNM3 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06334 1
EB06334 (5µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NIR1, PITPNM family member 3, PYK2 N-terminal domain-interacting receptor 1, RDGBA3, PITPNM3, CORD5, MGC157740, MGC157741, membrane-associated phosphatidylinositol transfer protein 3, retinal degeneration B alpha 3
Official Symbol: PITPNM3
Accession Number(s): NP_112497.2; NP_001159438.1
Human GeneID(s): 83394

Immunogen

Peptide with sequence C-SWARGPPKFESVP, from the C Terminus of the protein sequence according to NP_112497.2; NP_001159438.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 50kDa band in Human Heart lysates after 0.1µg/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_112497. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Cerebral Cortex shows strong staining of cytoplasm in several neuronal cells. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

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