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Goat Anti-NKG2D / KLRK1 Antibody - Trial (EB06839-T)

Related Products: EBP06839 - Immunizing Peptide, Goat Anti-NKG2D / KLRK1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06839-T Goat Anti-NKG2D / KLRK1 Antibody - Trial Pep-ELISA, FC, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite_Plus

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB06839-T 1
EB06839 Flow cytometric analysis of human peripheral blood monocytes. Primary incubation 30 minutes (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml).
EB06839-T 2
EB06839 staining (0.5ug/ml) of MOLT-4 cell lysate (A) + peptide (B). (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: KLRK1, NKG2-D, killer cell lectin-like receptor subfamily K, member 1, HGNC:18788, D12S2489E, KLR, NK cell receptor D, NKG2-D type II integral membrane protein, CD314, D12S2489E, FLJ17759, FLJ75772, NKG2D
Official Symbol: KLRK1
Accession Number(s): NP_031386.2
Human GeneID(s): 22914

Immunogen

Peptide with sequence C-KVYSKEDQDLLK, from the internal region of the protein sequence according to NP_031386.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 36-37kDa band observed in lysates of cell line MOLT4 (calculated MW of 25.3kDa according to NP_031386.2). This molecular weight is routinely observed by other sources, and was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-0.5µg/ml. Primary incubated 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis showing the expression of NKG2D on a subset of CD56+CD3- NK cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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