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Goat Anti-NKG2D / KLRK1 Antibody - Trial (EB06839-T)

Code Name Applications Availability Size Tested species Price Grade  
EB06839-T Goat Anti-NKG2D / KLRK1 Antibody - Trial Pep-ELISA, FC, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]

EB06839-T 1
EB06839 Flow cytometric analysis of human peripheral blood monocytes. Primary incubation 30 minutes (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml).
EB06839-T 2
EB06839 staining (0.5ug/ml) of MOLT-4 cell lysate (A) + peptide (B). (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: KLRK1, NKG2-D, killer cell lectin-like receptor subfamily K, member 1, HGNC:18788, D12S2489E, KLR, NK cell receptor D, NKG2-D type II integral membrane protein, CD314, D12S2489E, FLJ17759, FLJ75772, NKG2D
Official Symbol: KLRK1
Accession Number(s): NP_031386.2
Human GeneID(s): 22914


Peptide with sequence C-KVYSKEDQDLLK, from the internal region of the protein sequence according to NP_031386.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 36-37kDa band observed in lysates of cell line MOLT4 (calculated MW of 25.3kDa according to NP_031386.2). This molecular weight is routinely observed by other sources, and was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-0.5µg/ml. Primary incubated 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis showing the expression of NKG2D on a subset of CD56+CD3- NK cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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