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|EB05259-T||Goat Anti-NOS1 Antibody - Trial||Pep-ELISA, WB, IF, FC||20µg specific antibody in 40µl||Human||$105.00|
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Peptide with sequence C-ESKKDTDEVFSS, from the C Terminus of the protein sequence according to NP_000611.1; NP_001191147.1; NP_001191142.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: Strong expression of the protein seen in the nucleus and plasma membrane of U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
This antibody (previous batch) has been successfully used in IF on Mouse:
Fried HU, Kaupp UB, Müller F.
Hyperpolarization-activated and cyclic nucleotide-gated channels are differentially expressed in juxtaglomerular cells in the olfactory bulb of mice.
Cell Tissue Res. 2010 Mar;339(3):463-79.