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Goat Anti-Npap60 / Nup50 Antibody (EB05905)

Related Products: EBP05905 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05905 Goat Anti-Npap60 / Nup50 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05905 1
EB05905 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05905 2
EB05905 (3.8µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NUP50, NPAP60, NPAP60L, MGC39961, DKFZP564A043, nucleoporin 50kDa, nuclear pore-associated protein 60L, CTA-268H5.7, OTTHUMP00000199530
Official Symbol: NUP50
Accession Number(s): NP_009103.2; NP_705931.1
Human GeneID(s): 10762
Non-Human GeneID(s): 18141 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1).

Immunogen

Peptide with sequence C-ELHKILLEKKDA, from the C Terminus of the protein sequence according to NP_009103.2; NP_705931.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1µg/ml.
IHC: In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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