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Goat Anti-NPHS2 / SRN1 Antibody (EB12149)

Code Name Applications Availability Size Tested species Price Grade  
EB12149 Goat Anti-NPHS2 / SRN1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB12149 1
EB12149 (1µg/ml) staining of Human (A) and Mouse (B) Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NPHS2, nephrosis 2, idiopathic, steroid-resistant (podocin), PDCN, SRN1, podocin
Official Symbol: NPHS2
Accession Number(s): NP_055440.1
Human GeneID(s): 7827

Immunogen

Peptide with sequence C-SPSKPVEPLNPKK, from the C Terminus of the protein sequence according to NP_055440.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 42-45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to Human NP_055440.1 and Mouse NP_569723.1). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Pig, Cow

Specific Reference

This antibody (previous batch) has been successfully used in IF on Mouse:
Pepe M. Ekulu et al.
Novel Human Podocyte Cell Model Carrying G2/G2 APOL1 High-Risk Genotype
Cells. 2021 Aug; 10(8): 1914.
PMID: 34440683

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