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Goat Anti-NPR3 (aa520-533) Antibody (EB11190)

Related Products: EBP11190 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11190 Goat Anti-NPR3 (aa520-533) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB11190 1
EB11190 (1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11190 2
EB11190 (2µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
EB11190 3
EB11190 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
EB11190 4
EB11190 (10µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ANP-C, ANPRC, ANPR-C, atrial natriuretic peptide clearance receptor, atrial natriuretic peptide receptor 3, atrial natriuretic peptide receptor type C, GUCY2B, natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C), NPRC, NPR-C, OTTHUMP00000197048, OTTHUMP00000220980, NPR3
Official Symbol: NPR3
Accession Number(s): NP_000899.1
Human GeneID(s): 4883
Non-Human GeneID(s): 18162 (mouse), 25339 (rat)
Important Comments: The immunizing peptide represents the cytoplasmic part of the protein.

Immunogen

Peptide with sequence C-HRELREDSIRSHFS, from the C Terminus of the protein sequence according to NP_000899.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 52kDa band observed in Human Placenta lysates (calculated MW of 59.8kDa according to NP_000899.1). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Kidney shows staining of brush borders in select PCT. Recommended concentration: 2-4µg/ml. Paraffin embedded Human Prostate. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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