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Goat Anti-Nucleophosmin / NPM1 Antibody (EB06684)

Related Products: EBP06684 - Immunizing Peptide, Goat Anti-Nucleophosmin / NPM1 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB06684 Goat Anti-Nucleophosmin / NPM1 Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06684 1
EB06684 (1µg/ml) staining of NIH3T3 (A), (0.1ug/ml) Daudi (B), Jurkat (C) and (0.01ug/ml) K562 (D) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06684 2
EB06684 (1µg/ml) staining of Mouse Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06684 3
EB06684 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nucleoli staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06684 4
EB06684 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NPM1, NPM, B23, nucleophosmin (nucleolar phosphoprotein B23, numatrin), numatrin, nucleolar phosphoprotein B23, nucleophosmin/nucleoplasmin family, member 1, MGC104254, nucleophosmin 1
Official Symbol: NPM1
Accession Number(s): NP_001341935.1; NP_954654.1; NP_001341936.1; NP_001341939.1
Human GeneID(s): 4869

Immunogen

Peptide with sequence C-QEAIQDLWQWRKSL, from the C Terminus of the protein sequence according to NP_001341935.1; NP_954654.1; NP_001341936.1; NP_001341939.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 37kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and NIH3T3 and in Mouse Spleen lysates (calculated MW of 32.6kDa according to Human NP_001341935.1 and Mouse NP_032748.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nucleoli of HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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