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Goat Anti-ORC4L, Biotinylated Antibody (EB05067-B)

Related Products: EBP05067-B - Immunizing Peptide, EBP05067 - Immunizing Peptide, Goat Anti-ORC4L Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05067-B Goat Anti-ORC4L, Biotinylated Antibody Pep-ELISA, WB, ChIP Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05067-B 1
Biotinylated EB05067 (0.1µg/ml) staining of Jurkat nuclear lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: HSORC4, ORC4, origin recognition complex subunit 4, ORC4L, ORC4P, origin recognition complex, subunit 4 homolog, origin recognition complex, subunit 4 (yeast homolog)-like, Origin recognition complex, subunit 4, S. cerevisiae, homolog-like, origin recognition complex, subunit 4-like (yeast)
Official Symbol: ORC4
Accession Number(s): NP_002543.2; NP_001177810.1; NP_001177811.1
Human GeneID(s): 5000
Non-Human GeneID(s): 26428 (mouse), 295596 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_002543.2; NP_001177810.1; NP_001177811.1). Reported variants represent identical protein: XP_011509557.1, NP_002543.2, NP_001177808.1, NP_859526.1, NP_859525.1 Reported variants represent identical protein: XP_006712619.1, NP_001177810.1

Immunogen

Peptide with sequence C-DVRQWATSSLSWL., from the C Terminus of the protein sequence according to NP_002543.2; NP_001177810.1; NP_001177811.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 48kDa band observed in Jurkat nuclear lysates (calculated MW of 50.4kDa according to NP_002543.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Chromatin immunoprecipitation: reported by a customer to work very well in ChIP.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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