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Goat Anti-Osteopontin / SPP1 Antibody - Trial (EB06825-T)

Code Name Applications Availability Size Tested species Price Grade  
EB06825-T Goat Anti-Osteopontin / SPP1 Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]

EB06825-T 1
EB06825 (0.1µg/ml) staining of lysates of cell line MOLT4 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SPP1, HGNC:11255, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), BNSP, BSPI, ETA-1, MGC110940, OPN, Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), secreted phosphoprotein 1, SPP1/CALPHA1 fusion, bone sialoprotein I, early T-lymphocyte activation 1, osteopontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
Official Symbol: SPP1
Accession Number(s): NP_001035147.1; NP_000573.1; NP_001035149.1
Human GeneID(s): 6696
Non-Human GeneID(s): 20750 (mouse)


Peptide with sequence C-DPKSKEEDKHLK, from the C Terminus of the protein sequence according to NP_001035147.1; NP_000573.1; NP_001035149.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35kDa band observed in lysates of cell line MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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