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Goat Anti-OX1R and OX2R Antibody (EB08608)

Code Name Applications Availability Size Tested species Price Grade  
EB08608 Goat Anti-OX1R and OX2R Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB08608 1
EB08608 (0.5µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08608 2
EB08608 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: OX1R, OX2R, HCRTR1, hypocretin (orexin) receptor 1, hypocretin receptor 1, hypocretin receptor-1, orexin receptor 1, orexin receptor-1, HCRTR2, hypocretin (orexin) receptor 2, hypocretin receptor 2, hypocretin receptor-2, orexin receptor 2, orexin receptor-2
Official Symbol: HCRTR1
Accession Number(s): NP_001516.2; NP_001517.2
Human GeneID(s): 3061 , 3062
Non-Human GeneID(s): 230777 (mouse), 25593 (rat)
Important Comments: This antibody is expected to recognise both the human proteins OX1R and OX2R.

Immunogen

Peptide with sequence C-YNFLSGKFREQFK, from the internal region of the protein sequence according to NP_001516.2; NP_001517.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48kDa band observed in Human Brain (Frontal Cortex), Mouse Brain and Rat Brain lysates (calculated MW of 47.5kDa according to NP_001516.1 and NP_001517.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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