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|EB06163||Goat Anti-P114-RHO-GEF / ARHGEF18 Antibody||Pep-ELISA, WB, IF||100µg specific antibody in 200µl||Human||$392.00|
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Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2; NP_001124427.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 36404918.
This antibody has been successfully used in WB and IF on Human:
Reiko Satow, Yudai Suzuki, Shinobu Asada, Sae Ota, Masashi Idogawa, Shiori Kubota, Noi Ikeo, Atsuko Yoneda, Kiyoko Fukami
Downregulation of protein kinase C gamma reduces epithelial property and enhances malignant phenotypes in colorectal cancer cells.
iScience. 2022 Nov 4;25(12):105501.
This antibody (previous batch) has been successfully used in WB on Medaka:
Herder C, Swiercz JM, Müller C, Peravali R, Quiring R, Offermanns S, Wittbrodt J, Loosli F.
ArhGEF18 regulates RhoA-Rock2 signaling to maintain neuro-epithelial apico-basal polarity and proliferation.
Development. 2013 Jul;140(13):2787-97.
This antibody (previous batch) has been successfully used in WB on Human:
Terry SJ, Elbediwy A, Zihni C, Harris AR, Bailly M, Charras GT, Balda MS, Matter K.
Stimulation of Cortical Myosin Phosphorylation by p114RhoGEF Drives Cell Migration and Tumor Cell Invasion.
PLoS One. 2012;7(11):e50188.
This antibody (previous batch) has been successfully used in WB, IF and IP on Human:
Terry SJ, Zihni C, Elbediwy A, Vitiello E, Leefa Chong San IV, Balda MS, Matter K.
Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis.
Nat Cell Biol. 2011 Feb;13(2):159-66.