Change country
Home
Code Name Applications Availability Size Tested species Price Grade  
EB10589 Goat Anti-p47phox (mouse) Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB10589 1
EB10589 (1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10589 2
EB10589 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB10589 3
EB10589 Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: Ncf1, neutrophil cytosolic factor 1, NOXO2, Ncf-1, p47<phox>, p47phox, 47 kDa neutrophil oxidase factor, NADPH oxidase subunit (47 kD), NADPH oxidase subunit (47kDa), OTTMUSP00000022112, neutrophil NADPH oxidase factor 1, neutrophil cytosol facto
Official Symbol: Ncf1
Accession Number(s): NP_035006.3
Human GeneID(s): 653361
Non-Human GeneID(s): 17969 (mouse), 114553 (rat)

Immunogen

Peptide with sequence C-DEAEDPDPNYAGEP, from the internal region of the protein sequence according to NP_035006.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 48kDa band observed in lysates of cell line Daudi and in preliminary testing of Mouse Spleen lysate (calculated MW of 44.7kDa according to Human NP_000256.4. This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence:Expression of the protein seen in the cytoplasm and plasma membrane of NIH3T3 and RAW264.7 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Product Reviews

Please login to review this product.