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Goat Anti-PALS / MPP5 Antibody (EB06223)

Related Products: EBP06223 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06223 Goat Anti-PALS / MPP5 Antibody Pep-ELISA, IF, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06223 1
EB06223 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06223 2
EB06223 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06223 3
EB06223 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06223 4
B06223 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06223 5
EB06223 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: MPP5, PALS1, FLJ12615, membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5), stardust (Drosophila), MAGUK p55 subfamily member 5, membrane protein, palmitoylated 5, stardust
Official Symbol: PALS1
Accession Number(s): NP_071919.2; NP_001243479.1
Human GeneID(s): 64398

Immunogen

Peptide with sequence NKASPPFPLISNAQ-C, from the N Terminus of the protein sequence according to NP_071919.2; NP_001243479.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary testing showed a band at approx 75kDa in Human Cerebellum lysate after 1µg/ml antibody staining (calculated MW of 73.4kDa according to NP_001243479.1). Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Small Intestine and Kidney. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow, Dog, Pig

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