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Code Name Applications Availability Size Tested species Price Grade  
EB06599 Goat Anti-pan ADH Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB06599 1
EB06599 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: pan ADH, ADH1B, ADH2, alcohol dehydrogenase IB (class I), beta polypeptide, ADH, beta subunit, aldehyde reductase, alcohol dehydrogenase 2, alcohol dehydrogenase 2 (class I), beta polypeptide, alcohol dehydrogenase 1B (class I), beta polypeptide
Official Symbol: ADH1A, B, C
Accession Number(s): NP_000658.1; NP_000659.2; NP_000660.1
Human GeneID(s): 124 , 125
Important Comments: This antibody is expected to recognise the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of human alcohol dehydrogenase.

Immunogen

Peptide with sequence STAGKVMKCKA, from the N Terminus of the protein sequence according to NP_000658.1; NP_000659.2; NP_000660.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 38kDa band observed in Human Liver lysates (calculated MW of 39.9kDa according to NP_000658.1, NP_000659.2 and 000660.1). Recommended concentration: 1-3µg/ml.
IHC: Customer found this product to work in IHC on Human Liver.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Specific Reference

This antibody has been successfully used on Rat:
Yamashita H, Goto M, Matsui-Yuasa I, Kojima-Yuasa A.
Ecklonia cava Polyphenol Has a Protective Effect against Ethanol-Induced Liver Injury in a Cyclic AMP-Dependent Manner.
Mar Drugs. 2015 Jun 18;13(6):3877-91.
PMID: 26096275

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FAQs

Q: Why is there in the immunizing peptide a Methionine in the position of the Isoleucine according to ADH protein sequences?

A: At the time of design the protein sequence was not as well characterized and only later was the M replaced by the I

 

Q: Is there cross-reactivity of this antibody between the different ADH proteins?

A: Yes, for this is a pan-ADH antibody. It is meant to recognize all ADH proteins and isoforms in human.

Here is a match between the immunizing peptide (derived from the N-Terminus) with the different ADH proteins in Human:

EB06599:  STAGKVMKCKA
ADH1A:      STAGKVIKCKA
ADH1B:      STAGKVIKCKA
ADH1C:     STAGKVIKCKA
ADH4:        GTKGKVIKCKA
ADH5:         M-ANEVIKCKA
ADH6:        STTGQVIRCKA