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Goat Anti-Pancreatic Polypeptide / PPY Antibody (EB06805)

Code Name Applications Availability Size Tested species Price Grade  
EB06805 Goat Anti-Pancreatic Polypeptide / PPY Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06805 1
EB06805 (3µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer Ph9, HRP-staining.
EB06805 2
EB06805 (5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06805 3
EB06805 (5µg/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PPY, pancreatic polypeptide, HGNC:9327, PNP, pancreatic polypeptide Y
Official Symbol: PPY
Accession Number(s): NP_002713.1
Human GeneID(s): 5539


Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35-38kDa band in Human Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5kDa according to NP_002713.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3µg/ml. Paraffin embedded Human Pancreas and Intestine. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody has been successfully used in the following paper:
Hara A, Nakagawa Y, Nakao K, Tamaki M, Ikemoto T, Shimada M, Matsuhisa M, Mizukami H, Maruyama N, Watada H, Fujitani Y
Development of monoclonal mouse antibodies that speci€cally recognize pancreatic polypeptide
Endocr J. 2019 Mar 6. doi: 10.1507/endocrj.EJ18-0441.
PMID: 30842364

This antibody has been successfully used in IF on Human:
Abu Saleh Md Moin, Chiara Montemurro, Kylie Zeng, Megan Cory, Megan Nguyen, Shweta Kulkarni, Helga Fritsch, Juris J. Meier, Sangeeta Dhawan, Robert A. Rizza, Mark A. Atkinson and Alexandra E. Butler
Characterization of Non-hormone Expressing Endocrine Cells in Fetal and Infant Human Pancreas
Front. Endocrinol., 09 January 2019 |

This antibody has been successfully used in IF on Rat:
Md Moin AS, Dhawan S, Cory M, Butler PC, Rizza RA, Butler AE.
Increased Frequency of Hormone Negative  and Polyhormonal Endocrine Cells in Lean Individuals With Type 2 Diabetes.
J Clin Endocrinol Metab. 2016 Oct;101(10)
PMID: 27472443

This antibody has been successfully used in IHC on Human:
Butler AE, Dhawan S, Hoang J, Cory M, Zeng K, Fritsch H, Meier JJ, Rizza RA, Butler PC.
β-Cell Deficit in Obese Type 2 Diabetes, a Minor Role of β-Cell Dedifferentiation and Degranulation.
J Clin Endocrinol Metab. 2016 Feb;101(2):523-32.
PMID: 26700560

This antibody has been successfully used in Western blot on Human:
F. Aragón, M. Karaca, A. Novials, R.Maldonadoa, P. Maechler, and B. Rubí.
Pancreatic polypeptide regulates glucagon release through PPYR1 receptors expressed in mouse and human alpha-cells.
Biochim Biophys Acta. 2015 Feb;1850(2):343-51.
PMID: 25445712

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