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|EB06805||Goat Anti-Pancreatic Polypeptide / PPY Antibody||Pep-ELISA, IHC, IF||100µg specific antibody in 200µl||Human||$324.00|
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Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 30687234, and on Rat, PMID: 27472443.
This antibody has been successfully used in the following paper:
Hara A, Nakagawa Y, Nakao K, Tamaki M, Ikemoto T, Shimada M, Matsuhisa M, Mizukami H, Maruyama N, Watada H, Fujitani Y
Development of monoclonal mouse antibodies that specically recognize pancreatic polypeptide
Endocr J. 2019 Mar 6. doi: 10.1507/endocrj.EJ18-0441.
This antibody has been successfully used in IF on Human:
Abu Saleh Md Moin, Chiara Montemurro, Kylie Zeng, Megan Cory, Megan Nguyen, Shweta Kulkarni, Helga Fritsch, Juris J. Meier, Sangeeta Dhawan, Robert A. Rizza, Mark A. Atkinson and Alexandra E. Butler
Characterization of Non-hormone Expressing Endocrine Cells in Fetal and Infant Human Pancreas
Front. Endocrinol., 09 January 2019 | https://doi.org/10.3389/fendo.2018.00791
This antibody has been successfully used in IF on Rat:
Md Moin AS, Dhawan S, Cory M, Butler PC, Rizza RA, Butler AE.
Increased Frequency of Hormone Negative and Polyhormonal Endocrine Cells in Lean Individuals With Type 2 Diabetes.
J Clin Endocrinol Metab. 2016 Oct;101(10)
This antibody has been successfully used in IHC on Human:
Butler AE, Dhawan S, Hoang J, Cory M, Zeng K, Fritsch H, Meier JJ, Rizza RA, Butler PC.
β-Cell Deficit in Obese Type 2 Diabetes, a Minor Role of β-Cell Dedifferentiation and Degranulation.
J Clin Endocrinol Metab. 2016 Feb;101(2):523-32.
This antibody has been successfully used in Western blot on Human:
F. Aragón, M. Karaca, A. Novials, R.Maldonadoa, P. Maechler, and B. Rubí.
Pancreatic polypeptide regulates glucagon release through PPYR1 receptors expressed in mouse and human alpha-cells.
Biochim Biophys Acta. 2015 Feb;1850(2):343-51.