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Goat Anti-Pard3b (mouse) Antibody (EB10561)

Related Products: EBP10561 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10561 Goat Anti-Pard3b (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $423.00 Elite

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EB10561 1
EB10561 (0.5µg/ml) staining of Mouse fetal Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Pard3b, par-3 partitioning defective 3 homolog B (C. elegans), DN-276O13.2, 1810008K04Rik, 2010002N16Rik, 2810455B10Rik, Als2cr19, PAR3B, PAR3L, PAR3beta, RP23-20G7.1, DN-276O13.2, PAR3-L protein, PAR3-beta, amyotrophic lateral sclerosis 2 chromosomal region candidate gene 19 protein homolog, partitioning defective 3 homolog B, partitioning defective 3-like protein
Official Symbol: Pard3b
Accession Number(s): NP_001074519.2
Non-Human GeneID(s): 72823 (mouse), 301455 (rat)

Immunogen

Peptide with sequence C-RNQDPRQKNP, from the C-Terminus of the protein sequence according to NP_001074519.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 125kDa band observed in Mouse fetal Kidney lysates (calculated MW of 133kDa according to NP_001074519.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Mouse, Rat

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