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Goat Anti-PARP4 / VPARP Antibody (EB07100)

Related Products: EBP07100 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07100 Goat Anti-PARP4 / VPARP Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
Click here to download PDF datasheet.

Target Protein

Principal Names: PARP4, poly (ADP-ribose) polymerase family, member 4, HGNC:271, ADPRTL1, PARPL, PH5P, VAULT3, VPARP, p193, ADP-ribosyltransferase (NAD poly ADP-ribose polymerase)-like 1, H5 proline-rich I-alpha-I-related, OTTHUMP00000042288, PARP-related, poly(ADP-ribose) synthetase, poly(ADP-ribosyl)transferase-like 1, vault protein, 193-kDa
Official Symbol: PARP4
Accession Number(s): NP_006428.1
Human GeneID(s): 143

Immunogen

Peptide with sequence DTHELKQKRTDC, from the internal region of the protein sequence according to NP_006428.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 80-85kDa band in Human Tonsil and HeLa lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 193kDa according to NP_006428.1. The 80-85kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Dog

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