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Code Name Applications Availability Size Tested species Price Grade  
EB11338 Goat Anti-PAX3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11338 1
EB11338 (2µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CDHS, HUP2, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, paired box 3, paired box homeotic gene 3, paired domain gene 3, paired domain gene HuP2, WS1, PAX3
Official Symbol: PAX3
Accession Number(s): NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1
Human GeneID(s): 5077
Non-Human GeneID(s): 18505 (mouse), 114502 (rat)
Important Comments: This antibody is expected to recognize isoforms PAX3 (NP_852122.1), PAX3d (NP_852123.1), PAX3e (NP_852124.1), PAX3g (NP_852126.1), PAX3h (NP_852125.1), and PAX3i (NP_001120838.1).

Immunogen

Peptide with sequence C-STVHQSTIPSNPD, from the internal region of the protein sequence according to NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55kDa band observed in Human Brain (Frontal Cortex) and Mouse and Rat Brain lysates (calculated MW of 53.0kDa according to Human NP_852122.1 and 52.9kDa according to Mouse NP_032807.3). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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