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Goat Anti-PAX6 (internal) Antibody (EB07772)

Related Products: EBP07165 - Immunizing Peptide, EBP07772 - Immunizing Peptide, Goat Anti-PAX6 (C Terminus) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07772 Goat Anti-PAX6 (internal) Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07772 1
EB07772 (0.3µg/ml) staining of U251 (A), (0.03ug/ml) nuclear K562 (B) and (1ug/ml) nuclear HeLa (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07772 2
.EB07772 (0.5µg/ml) staining of RAW264.7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07772 3
EB07772 Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PAX6, paired box gene 6 (aniridia, keratitis), AN, AN2, D11S812E, MGC17209, MGDA, WAGR, paired box gene 6, paired box homeotic gene 6 (aniridia, keratitis), paired box homeotic gene-6
Official Symbol: PAX6
Accession Number(s): NP_000271.1; NP_001595.2; NP_001297088.1; NP_001355839.1; NP_001355840.1; NP_001355841.1; NP_001355844.1; NP_001355847.1; NP_001355849.1; NP_001355850.1; NP_001355851.1; NP_001355857.1; NP_001355858.1; NP_001355859.1
Human GeneID(s): 5080
Non-Human GeneID(s): 18508 (mouse), 25509 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms: NP_000271.1; NP_001595.2; NP_001297088.1; NP_001355839.1; NP_001355840.1; NP_001355841.1; NP_001355844.1; NP_001355847.1; NP_001355849.1; NP_001355850.1; NP_001355851.1; NP_001355857.1; NP_001355858.1; NP_001355859.1.

Immunogen

Peptide with sequence C-REEKLRNQRRQASN, from the internal region of the protein sequence according to NP_000271.1; NP_001595.2; NP_001297088.1; NP_001355839.1; NP_001355840.1; NP_001355841.1; NP_001355844.1; NP_001355847.1; NP_001355849.1; NP_001355850.1; NP_001355851.1; NP_001355857.1; NP_001355858.1; NP_001355859.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26kDa band observed in lysates of cell lines U251 and RAW264.7 and in nuclear lysates of cell lines K562 and HeLa, all successfully blocked by incubation with the immunising peptide (calculated MW of 24.8kDa according to Human NP_001355859.1 and 31.8 according to Mouse NP_001297075.1). Recommended concentration: 0.03-1µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Fog, Cow

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