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|EB05513||Goat Anti-PAX8 (N Terminus) Antibody||Pep-ELISA, WB, IHC||100µg specific antibody in 200µl||Human||$324.00|
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Peptide with sequence PHNSIRSGHGGLN-C, from the N Terminus of the protein sequence according to NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Message from a distributor:
Please could you assist with this technical issue. The customer saw positive staining in the membrane of catalog# EB05513 . Please could you suggest a cause of this. Can you recommend a dilution and enzyme digestion?
Our EB05513 reacts with transcription factor PAX8 and it should be staining the nuclei. This is exactly what we have found in Human Thyroid Gland. It is possible however that this product does not work properly after different epitope retrieval methods. We obtained our IHC data after microwaved antigen retrieval with Tris/EDTA at pH9. A different pH value or a different heating type may result in different results.
I hope this will help your customer further. Please let me know when problems persist after adjustment of the epitope retrieval method.