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Goat Anti-PAX8 (N Terminus) Antibody (EB05513)

Code Name Applications Availability Size Tested species Price Grade  
EB05513 Goat Anti-PAX8 (N Terminus) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
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EB05513 1
EB05513 (1µg/ml) staining of Human Kidney (A) and HeLa cell (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05513 2
EB05513 (5µg/ml) staining of paraffin embedded Human Kidney Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PAX8, paired box gene 8, paired domain gene 8, paired box 8
Official Symbol: PAX8
Accession Number(s): NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1
Human GeneID(s): 7849
Non-Human GeneID(s): 18510 (mouse), 81819 (rat)
Important Comments: This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)

Immunogen

Peptide with sequence PHNSIRSGHGGLN-C, from the N Terminus of the protein sequence according to NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 35kDa band observed in Human Kidney lysates. An additional 48kDa was also observed in some Kidney and HeLa cell lysates (calculated MW of 34.9kDa according to Human NP_039247.1 PAX8D and 48.2kDa according to Human NP_003457.1 PAXA). Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and Rat Liver, and NIH3T3 for this particular batch.
IHC: Paraffin embedded Human Kidney Cortex. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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FAQs

 


Message from a distributor:

Please could you assist with this technical issue.  The customer saw positive staining in the membrane of catalog# EB05513 .  Please could you suggest a cause of this. Can you recommend a dilution and enzyme digestion?

Everest Reply:

Our EB05513 reacts with transcription factor PAX8 and it should be staining the nuclei. This is exactly what we have found in Human Thyroid Gland. It is possible however that this product does not work properly after different epitope retrieval methods. We obtained our IHC data after microwaved antigen retrieval with Tris/EDTA at pH9. A different pH value or a different heating type may result in different results.

I hope this will help your customer further. Please let me know when problems persist after adjustment of the epitope retrieval method.