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Goat Anti-PCQAP / MED15 Antibody (EB09173)

Related Products: EBP09173 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09173 Goat Anti-PCQAP / MED15 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09173 1
EB09173 (1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MED15, mediator complex subunit 15, ARC105, CAG7A, CTG7A, DKFZp686A2214, DKFZp762B1216, FLJ42282, FLJ42935, PCQAP, TIG-1, TIG1, TNRC7, CTG repeat protein 7a, PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein, PC2-glutamine-rich-associated protein, TPA inducible gene-1, TPA inducible protein, activator-recruited cofactor, 105-kD, positive cofactor 2, glutamine/Q-rich-associated protein, trinucleotide repeat containing 7
Official Symbol: MED15
Accession Number(s): NP_001003891.1; NP_056973.2
Human GeneID(s): 51586
Non-Human GeneID(s): 94112 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001003891.1; NP_056973.2).

Immunogen

Peptide with sequence C-PPQSQTQPLVSQAQ, from the internal region of the protein sequence according to NP_001003891.1; NP_056973.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 90kDa band observed in Human Lung lysates (calculated MW of 86.8kDa according to NP_001003891.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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