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Goat Anti-PDIA3 / ERp57 / GRP58 Antibody - Trial (EB05735-T)

Related Products: EBP05735 - Immunizing Peptide, Goat Anti-PDIA3 / ERp57 / GRP58 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05735-T Goat Anti-PDIA3 / ERp57 / GRP58 Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human, Mouse $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05735-T 1
EB05735 staining (0.1µg/ml) of Daudi lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PDIA3, GRP58, ERp57, protein disulfide isomerase family A, member 3, ERp60, ERp61, GRP57, HsT17083, P58, PI-PLC, 58 kDa microsomal protein, endoplasmic reticulum P58, glucose regulated protein, 58kDa, phospholipase C-alpha, protein disulfide isomerase-associated 3, ER60, protein disulfide-isomerase A3
Official Symbol: PDIA3
Accession Number(s): NP_005304.3
Human GeneID(s): 2923
Non-Human GeneID(s): 14827 (mouse), 29468 (rat)

Immunogen

Peptide with sequence C-EEKPKKKKKAQEDL, from the C Terminus of the protein sequence according to NP_005304.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 60kDa band observed in lysates of cell lines NIH3T3, HepG2 and Daudi (calculated MW of 56.8kDa according to NP_005304.3). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was for 1 hour.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow

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