Change country
Home

Goat Anti-Perilipin (C Terminus) Antibody (EB07728)

Code Name Applications Availability Size Tested species Price Grade  
EB07728 Goat Anti-Perilipin (C Terminus) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07728 1
EB07728 (0.5µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07728 2
EB07728 (4µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB07728 3
EB07728 staining of differentiated 3T3-L1 adipocytes. Data kindly provided by Prof. J. Granneman, Detroit, USA. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: Plin, perilipin, PERI, lipid droplet-associated protein
Official Symbol: PLIN
Accession Number(s): NP_002657.2
Human GeneID(s): 5346
Non-Human GeneID(s): 25629 (rat)

Immunogen

Peptide with sequence C-EPILGRAQYSQLRKK, from the C Terminus of the protein sequence according to NP_002657.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55kDa band observed in Human Adipose lysate (calculated MW of 56.0kDa according to NP_002657.2). Recommended concentration: 0.3-1 µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog

Specific References

This antibody (previous batch) has been successfully used in IF on Human:
Emilio P.Mottillo, Huamei Zhang, Alexander Yang, Li Zhou, James G.Granneman
Genetically-encoded sensors to detect fatty acid production and trafficking
Molecular Metabolism, Vol 29, Nov. 2019, https://doi.org/10.1016/j.molmet.2019.08.012
PMID: 0

This antibody (previous batch) has been successfully used in IHC and IF on Mouse:
Lee YH, Petkova AP, Konkar AA, Granneman JG.
Cellular origins of cold-induced brown adipocytes in adult mice.
FASEB J. 2015 Jan;29(1):286-99.
PMID: 25392270

Product Reviews

Please login to review this product.