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Goat Anti-Peripherin 1 (mouse) Antibody (EB12405)

Related Products: EBP12405 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12405 Goat Anti-Peripherin 1 (mouse) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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www.arp1.com
EB12405 1
EB12405 (0.1µg/ml) staining of Rat Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Prph, peripherin, Prph1, peripherin 1
Official Symbol: Prph
Accession Number(s): NP_038667.2; NP_001157060.1; NP_001157061.1
Non-Human GeneID(s): 19132 (mouse), 24688 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_038667.2; NP_001157060.1; NP_001157061.1).

Immunogen

Peptide with sequence C-QKEQHSDLDKSSIH, from the C Terminus of the protein sequence according to NP_038667.2; NP_001157060.1; NP_001157061.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55kDa band observed in Rat Colon lysates (calculated MW of 54.0kDa according to NP_036765.2). Recommended concentration: 0.1-0.3µg/ml. An additional faint band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.
IHC: This antibody has been successfully used in IHC on Mouse, PMID: 36226085.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse, Rat

Specific Reference

This antibody has been successfully used in IHC on Mouse:
Jennie M E Cederholm, Kristina E Parley, Chamini J Perera, Georg von Jonquieres, Jeremy L Pinyon, Jean-Pierre Julien, David K Ryugo, Allen F Ryan, Gary D Housley
Noise-induced hearing loss vulnerability in type III intermediate filament peripherin gene knockout mice.
Front Neurol. 2022 Sep 26;13:962227.
PMID: 36226085

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