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Goat Anti-Pescadillo Antibody (EB05994)

Related Products: EBP05994 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05994 Goat Anti-Pescadillo Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05994 1
EB05994 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05994 2
EB05994 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PES1, pescadillo homolog 1, containing BRCT domain (zebrafish), PES, pescadillo (zebrafish) homolog 1, containing BRCT domain
Official Symbol: PES1
Accession Number(s): NP_055118.1
Human GeneID(s): 23481
Non-Human GeneID(s): 64934 (mouse)

Immunogen

Peptide with sequence GGLEKKKYERGSA-C, from the N Terminus of the protein sequence according to NP_055118.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70kDa band observed in HeLa lysate (calculated MW of 68kDa according to NP_055118). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Testis shows nucleolar staining in spermatocytes and spermatids. Recommended concentration, 2.5-3.8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Pig, Cow

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