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Goat Anti-PGAM1 / PGAM2 / PGAM4 Antibody (EB05028)

Code Name Applications Availability Size Tested species Price Grade  
EB05028 Goat Anti-PGAM1 / PGAM2 / PGAM4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $315.00 Elite

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EB05028 1
EB05028 (0.05µg/ml) staining of Human (A), Mouse (B), Rat (C) and Pig Liver (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PGAM1, PGAM2, phosphoglycerate mutase 1 (brain), PGAMA, RP11-452K12.8, OTTHUMP00000059414, Phosphoglycerate mutase A, nonmuscle form, phosphoglycerate mutase 2 (muscle), MGC88743, PGAMM, Phosphoglycerate mutase, muscle form, PGAM3, phosphoglycerate mutase family member 4, PGAM-B, dJ1000K24.1, phosphoglycerate mutase family 3, phosphoglycerate mutase family 4, phosphoglycerate mutase processed protein, bisphosphoglycerate mutase 1, bisphosphoglycerate mutase 2, bisphosphoglycerate mutase 4, BPG-dependent PGAM 1, OTTHUMP00000020190, PGAM-M, BPG-dependent PGAM 2, OTTHUMP00000063546
Official Symbol: PGAM1 / PGAM2 / PGAM4
Accession Number(s): NP_002620.1; NP_000281.2; NP_001025062.1
Human GeneID(s): 5223 , 5224
Important Comments: Please note this antibody is expected to recognize the products of 3 highly similar genes.

Immunogen

Peptide with sequence C-KAMEAVAAQGKAKK, from the C Terminus of the protein sequence according to NP_002620.1; NP_000281.2; NP_001025062.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 28kDa band observed in Human, Mouse, Rat and Pig Liver lysates (calculated MW of 28.8kDa according to NP_002620.1; NP_000281.2 and of NP_001025062.1). The additional 30kDa band observed in the rodent lysates likely represent the acetylated form. Recommended concentration: 0.05-0.15µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody (previous batch) has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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