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Goat Anti-PGRMC1 / MPR Antibody (EB07207)

Related Products: EBP07207 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07207 Goat Anti-PGRMC1 / MPR Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07207 1
EB07207 (0.1µg/ml) staining of Human Kidney (A), Liver (B) and Testes (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07207 2
EB07207 (0.1µg/ml) staining of Mouse Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07207 3
EB07207 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PGRMC1, MPR, progesterone receptor membrane component 1, HGNC:16090, HPR6.6, progesterone binding protein
Official Symbol: PGRMC1
Accession Number(s): NP_006658.1
Human GeneID(s): 10857
Non-Human GeneID(s): 53328 (mouse)

Immunogen

Peptide with sequence C-EPKDESARKND, from the C Terminus of the protein sequence according to NP_006658.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 24kDa band observed in Human Kidney, Liver and Testis lysates, and approx 25kDa in Mouse Testis lysates (calculated MW of 21.7kDa according to Human NP_006658.1 and Mouse NP_058063.2). These molecular weights are routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.

Immunofluorescence: This antibody has been successfully used in IF on Human: Izzo, N. J et al. (2014) PMID: 25390692.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

Specific Reference

This antibody has been successfully used in IF on Human:
Izzo, N. J., Xu, J., et al.
Alzheimer's therapeutics targeting amyloid beta 1-42 oligomers II: Sigma-2/PGRMC1 receptors mediate Abeta 42 oligomer binding and synaptotoxicity.
PLoS One. 2014 Nov 12;9(11):e111899.
PMID: 25390692

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