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Goat Anti-PHR1 Antibody (EB09008)

Related Products: EBP09008 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09008 Goat Anti-PHR1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09008 1
EB09008 (1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PLEKHB1, pleckstrin homology domain containing, family B (evectins) member 1, KPL1, PHR1, PHRET1, PH domain containing protein in retina 1, PH domain containing, retinal 1, evectin-1
Official Symbol: PLEKHB1
Accession Number(s): NP_067023.1; NP_001123506.1; NP_001123505.1; NP_001123507.1; NP_001123508.1;
Human GeneID(s): 58473
Non-Human GeneID(s): 27276 (mouse), 64471 (rat)
Important Comments: This antibody is expected to recognize reported isoforms a (NP_067023.1) and c (NP_001123505.1).

Immunogen

Peptide with sequence PPDSALESPFEE-C, from the N Terminus of the protein sequence according to NP_067023.1; NP_001123506.1; NP_001123505.1; NP_001123507.1; NP_001123508.1;.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 25kDa band observed in Rat Brain lysates (calculated MW of 25.2kDa according to Rat NP_742030.1 (Evectin-1) and of 27.2kDa according to Human NP_067023.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Cow

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