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Code Name Applications Availability Size Tested species Price Grade  
EB10892 Goat Anti-PIM2 (aa23-36) Antibody Pep-ELISA, WB-Trf, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10892 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with EB10892 (0.1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB10892 (0.1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
EB10892 2
EB10892 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PIM2, pim-2 oncogene, pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2
Official Symbol: PIM2
Accession Number(s): NP_006866.2
Human GeneID(s): 11040
Non-Human GeneID(s): 18715 (mouse), 317366 (rat)

Immunogen

Peptide with sequence C-KDREAFEAEYRLGP, from the internal region (near N Terminus) of the protein sequence according to NP_006866.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration: 0.5-1µg/ml.
IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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