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Code Name Applications Availability Size Tested species Price Grade  
EB10834 Goat Anti-PIM2 Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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EB10834 1
EB10834 (1µg/ml) staining of HepG2 (A) and K562 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10834 2
EB10834 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB10834 3
EB10834 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB10834 4
EB10834 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PIM2, pim-2 oncogene, pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2
Official Symbol: PIM2
Accession Number(s): NP_006866.2
Human GeneID(s): 11040

Immunogen

Peptide with sequence C-QTPAEDVPLNPSK, from the C Terminus of the protein sequence according to NP_006866.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 30kDa band observed in lysates of cell line K562 and in preliminary testing of cell lines HeLa and Caco-2 and approx. 30-35kDa observed in lysates of cell line HepG2 (calculated MW of 34.2kDa according to NP_006866.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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