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Goat Anti-Pirin Antibody (EB06437)

Related Products: EBP05026 - Immunizing Peptide, EBP06437 - Immunizing Peptide, Goat Anti-Pirin Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06437 Goat Anti-Pirin Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06437 1
EB06437 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06437 2
EB06437 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06437 3
EB06437 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: pirin, PIR, pirin (iron-binding nuclear protein)
Official Symbol: PIR
Accession Number(s): NP_003653.1
Human GeneID(s): 8544
Important Comments: Variants (NP_003653.1; NP_001018119.1) encode the same protein

Immunogen

Peptide with sequence GSSKKVTLSVLSREC, from the N Terminus of the protein sequence according to NP_003653.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 33kDa band observed in Human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1). Recommended concentration: 0.3 - 1.0 µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Liver and Spleen. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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