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Code Name Applications Availability Size Tested species Price Grade  
EB05500-T Goat Anti-Plakoglobin / Gamma-catenin Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human, Mouse, Rat $105.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05500-T 1
EB05500 (0.3µg/ml) staining of A431 (A) and NIH3T3 (B) cell lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05500-T 2
EB05500 staining (1µg/ml) of Rat Eye lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05500-T 3
EB05500 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: JUP, GAMMA-CATENIN, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa, desmoplakin III, desmoplakin-3
Official Symbol: JUP
Accession Number(s): NP_002221.1, NP_068831.1
Human GeneID(s): 3728
Non-Human GeneID(s): 16480 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Immunogen

Peptide with sequence C-GLRPPYPTADHMLA, from the C Terminus of the protein sequence according to NP_002221.1, NP_068831.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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